Collagen stimulates tyrosine phosphorylation of phospholipase C-γ2 but not phospholipase C-γ1 in human platelets
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چکیده
منابع مشابه
Tyrosine phosphorylation is involved in receptor coupling to phospholipase D but not phospholipase C in the human neutrophil.
The tyrosine kinase inhibitors ST271, ST638 and erbstatin inhibited phospholipase D (PLD) activity in human neutrophils stimulated by fMet-Leu-Phe, platelet-activating factor and leukotriene B4. These compounds did not inhibit phorbol ester-stimulated PLD, indicating that they do not inhibit PLD per se, but probably act at a site between the receptor and the phospholipase. In contrast, the prot...
متن کاملTyrosine phosphorylation regulates phospholipase C activation in human T-cells.
Introduction One early transmembrane signal in response to stimulation of the T-cell antigen receptor (CD 3/Ti) is the activation of phosphatidylinositol (Ptd1ns)specific phospholipase C (PLC). The resulting formation of inositol 1,4,5-trisphosphate (Ins( 1,4,5)q?) and diacylglycerol (DAG) causes calcium release from internal stores, calcium influx through calcium channels, and translocation of...
متن کاملParkin-mediated ubiquitination regulates phospholipase C-γ1
Mutations in parkin cause autosomal recessive forms of Parkinson's disease (PD), with an early age of onset and similar pathological phenotype to the idiopathic disease. Parkin has been identified as an E3 ubiquitin ligase that mediates different types of ubiquitination, which has made the search for substrates an intriguing possibility to identify pathological mechanisms linked to PD. In this ...
متن کاملActivation of Phospholipase C 2 by Tyrosine Phosphorylation
Phospholipase C 2 (PLC 2) has been implicated in collageninduced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLC 2. We expressed the full-length cDNA for human PLC 2 in bacteria and purified the recombinant enzyme. The recombinant enzyme was Ca -dependent with optimal activity in the range of 1 to 10 M C...
متن کاملPhospholipase A 2 and Phospholipase C Activities of Platelets DIFFERENTIAL
Deoxycholate treatment of horse platelets previously labeled in their phospholipids with [“Clarachidonate produces selective conversion of [“Clphosphatidylinositol (PI) to [‘4C]1,2-diacylglycerol. This phospholipase C activity, which has a pH optimum of 7.5, is specific for phosphatidylinositol since other phospholipids or neutral lipids are not affected. Although exogenous Ca2+ is not required...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1994
ISSN: 0014-5793
DOI: 10.1016/0014-5793(94)01037-4